Rita Perlingeiro, Ph.D.

Professor of Medicine, Cardiovascular Division

E-MAIL: perli032@umn.edu

Research Interests:

Our laboratory has a long-term interest in understanding the molecular mechanisms controlling lineage-specific differentiation of pluripotent stem cells (i.e. embryonic and adult reprogrammed stem cells), and applying this information to efficiently generate tissue-specific stem/progenitor cells endowed with in vivo regenerative potential. Our ultimate goal is to develop safe strategies to enable their future therapeutic application.

Selected Publications:

  • Chan SS, Arpke RW, Filareto A, Xie N, Pappas MP, Penaloza JS, Perlingeiro RCR, Kyba M. Skeletal muscle stem cells from PSC-derived teratomas have functional regenerative capacity. Cell Stem Cell. 2018 Jul 5;23(1):74-85.e6.
  • Mondragon-Gonzalez R, Perlingeiro RCR. Recapitulating muscle disease phenotypes with myotonic dystrophy 1 induced pluripotent stem cells: a tool for disease modeling and drug discovery. Dis Model Mech. 2018 Jul 18;11(7). pii: dmm034728.
  • Kim, J, Oliveira VKP, Yamamoto A, Perlingeiro RCR. Generation of skeletal myogenic progenitors from human pluripotent stem cells using non-viral delivery of minicircle DNA. Stem Cell Res. 2017;23:87-94.
  • Magli A, Incitti T, Kiley J, Swanson SA, Darabi R, Rinaldi F, Selvaraj S, Yamamoto A, Tolar J, Yuan C, Stewart R, Thomson JA, Perlingeiro RCR. PAX7 targets, CD54, integrin α9β1, and SDC2, allow isolation of human ESC/iPSC-derived myogenic progenitors. Cell Rep. 2017;19:2867-2877.
  • Kim J, Magli A, Chan SSK, Oliveira VKP, Wu J, Darabi R, Kyba M, Perlingeiro RCR. Expansion and purification are critical for the therapeutic application of pluripotent stem cell-derived myogenic progrenitors. Stem Cell Reports 2017;9:12-22.
  • Dourado KC, Baik J, Oliveira VKP, Beltrame M, Yamamoto A, Theuer, CP, Figueiredo CAV, Verneris M, Perlingeiro RCR. Endoglin: a novel target for therapeutic intervention in acute leukemias revealed in xenograft mouse models. Blood. 2017;129:2526-2536.
  • Baik J, Magli A, Tahara N, Swanson SA, Borges L, Koyano-Nakagawa N, Stewart R, Garry DG, Kawakami Y, Thomson JA, Perlingeiro RCR. Endoglin integrates BMP and Wnt signaling to induce hematopoiesis through JDP2. Nat Commun. 2016;7:13101.
  • Carrió E, Magli A, Muñoz M, Peinado MA, Perlingeiro RCR, Suelves M Muscle cell identity requires Pax7-mediated lineage-specific DNA demethylation. BMC Biol. 2016;14:30.
  • Filareto A, Rinaldi F, Arpke RW, Darabi R, Belanto JJ, Toso EA, Miller AZ, Ervasti JM, McIvor RS, Kyba M, Perlingeiro RCR. Pax3-induced expansion enables the genetic correction of dystrophic satellite cells. Skelet Muscle 2015;5:36.
  • McCullagh KJA, Perlingeiro RCR. Coaxing stem cells for skeletal muscle repair. Adv Drug Deliv Rev. 2015;84:198-207.
  • Skoglund G, Laine J, Darabi R, Fournier E, Perlingeiro RCR, Tabti N. Physiological and ultrastructural features of human induced pluripotent and embryonic stem cell-derived skeletal myocytes in vitro. Proc Natl Acad Sci USA 2014;111:8275-8280.
  • Magli A, Schnettler E, Swanson SA, Borges L, Hoffman K, Stewart R, Thomson JA, Perlingeiro RCR. Pax3 and Tbx5 specify whether PDGFR?+ cells assume skeletal or cardiac muscle fate in differentiating ES cells. Stem Cells. 2014;32:2072-2083.
  • Filareto A, Parker S, Darabi R, Borges L, Iacovino M, Schaaf T, Mayerhofer T, Chamberlain JS, Ervasti JM, McIvor RS, Kyba M, Perlingeiro RCR. An ex vivo eene therapy approach to treat muscular dystrophy using inducible pluripotent stem cells. Nat Commun. 2013;4:1549.
  • Magli A, Schnettler E, Rinaldi F, Brember P, Perlingeiro RCR. (2013) Functional dissection of Pax3 in paraxial mesoderm development and myogenesis. Stem Cells. 2013;31(1):59-60.
  • Borges L, Iacovino M, Mayerhofer T, Koyano-Nakagawa N, Baik J, Garry DJ, Kyba M, Letarte M, Perlingeiro RCR. A critical role for endoglin in the emergence of blood during embryonic development. Blood. 2012;119(23):5417-5428.
  • Darabi R, Arpke RW, Irion S, Dimos JT, Grskovic M, Kyba M, Perlingeiro RCR. Human ES- and iPS-derived myogenic progenitors restore dystrophin and improve contractility upon transplantation in dystrophic mice. Cell Stem Cell. 2012;10(5):610-619.

Current Graduate Students:

Bayardo Garay (Neuroscience, University of Minnesota).